In-Vitro study of the Anticoagulant Property of Terminalia Catappa (Talisay) Leaf Extract Using Gallus Gallus (Chicken) Blood

  • Vandon T. Borela Teacher, Parang High School, the Philippines
  • Klim Angelo L. Balunsat Students, Parang High School, the Philippines
  • Alyssa Juliana L. Briones Students, Parang High School, the Philippines
  • Patricia Nicole P. Briones Students, Parang High School, the Philippines
Keywords: Blood Coagulation, Anticoagulation, Prothrombin Time, Herbal Medicine, erminalia Catappa (Talisay)


Prothrombin time is a test used to help detect and diagnose a bleeding disorder or excessive clotting disorder. It is important to know the prothrombin time because it checks to see if five different blood clotting factors are present. Lack of Vitamin K and having Liver problems are one of the factors that can decrease the prothrombin time of the blood. This research aims to investigate the potential ability of the Terminalia catappa  (Talisay) leaf extract to increases prothrombin time of chicken’s blood. The research methods used by the researchers is experimental. A research design where the results are being observed and determined when the independent variable took effect on the dependent variable. . The materials used are ethanol, calcium chloride, leaves and chicken blood. The method that was used in the study is in-vitro, a process that is performed in a test tube, or elsewhere outside a living organism.  The 0.5 ml plasma sample was separated into four tubes and the first group of plasma was tested first to determine the initial prothrombin time while the other three groups with the different volumes of plant extract (0.2, 0.4, 0.6 mg/ml)  was added separately. The tubes were tilted at about 45° until the permanent clots was obtained. These are recorded as the prothrombin time. The research showed that the Terminalia Catappa leaf extract is effective on increasing the normal prothrombin time of the blood.


Asha, S. (2017). In vitro anticoagulant activity of Nelumbo nucifera leaf extracts on normal healthy blood plasma. International Journal of Green Pharmacy (IJGP), 11(03).

Ayodele, O. O., Onajobi, F. D., & Osoniyi, O. (2019). In vitro anticoagulant effect of Crassocephalum crepidioides leaf methanol extract and fractions on human blood. Journal of experimental pharmacology, 11, 99.

Benjamin, E. J., Blaha, M. J., Chiuve, S. E., Cushman, M., Das, S. R., Deo, R., & Isasi, C. R. (2017). Heart disease and stroke statistics—2017 update.

Chegu, K., Mounika, K., Rajeswari, M., Vanibala, N., Sujatha, P., Sridurga, P., & Reddy, D. B. (2018). In vitro study of the anticoagulant activity of some plant extracts. World J Pharm Pharm Sci, 7(5), 904-13.

Davidson, I., & Henry, J. B. (1969). Clinical diagnosis by laboratory methods. Philadelphia, PA: Saunders.

Gou, Y. L., Ho, A. L. S., Rowlands, D. K., Chung, Y. W., & Chan, H. C. (2003). Effects of Bak Foong Pill on blood coagulation and platelet aggregation. Biological and Pharmaceutical Bulletin, 26(2), 241-246.

Mgbemene, C. N., & Ohiri, F. C. (1999). Anti-sickling potential of Terminalia catappa leaf extract. Pharmaceutical biology, 37(2), 152-154.

Muhammad, A., & Mudi, Y. (2011). Phytochemical screening and antimicrobial activities of Terminalia catappa, leaf extracts. Biokemistri, 23(1).

Offor, C. E., Ugwu, P. C., Okechukwu, P. M., & Igwenyi, I. O. (2015). Proximate and phytochemical analyses of Terminalia catappa leaves. European Journal of Applied Sciences, 7(1), 09-11.

Padh, H., & Patel, B. (2001). Invitro Anticoagulant Activity of Allium Sativum Plant Extract. World Journal of Pharmacy and Pharmaceutical Sciences. 6647.1256-1261. 10.20959/wjpps20179-10017

Sox, H. C. (1990). Common diagnostic tests: use and interpretation. Amer College of Physicians.

How to Cite
Borela, V. T., Balunsat, K. A. L., Briones, A. J. L., & Briones, P. N. P. (2020). In-Vitro study of the Anticoagulant Property of Terminalia Catappa (Talisay) Leaf Extract Using Gallus Gallus (Chicken) Blood. Journal La Lifesci, 1(3), 25-29.