Journal La Lifesci 2021-04-30T09:52:11+07:00 Andrew Setiawan Rusdianto Open Journal Systems <p>International <strong>Journal La </strong><strong>Lifesci</strong>&nbsp; ISSN 2721-1207 (online) and ISSN 2721-1304 (Print) includes all the areas of research activities in all fields of Life Sciences such as Agricultural, Fisheries, Earth, Environmental Science, Botany, Zoology, Microbiology, Ecology, Ethnobiology, Biodiversity And Conservation Biology, Genetics, Biochemistry , Bioinformatics, Biophysics, Biostatistics, Cytobiology Developmental Biology, Entomology, Immunology , Molecular Biology, Virology, agronomy, plant and animal breeding, agricultural economics and rural sociology,&nbsp; Veterinary science, Ornithology, Primatology, Biogeography, Histology, Marine biology, Biochemical Sciences Aquaculture, Fishery Hydrography, Fishery Engineering, Aquatic ecosystem, Fish farming, Fisheries management, Fishery Biology, Wild fisheries, Ocean fisheries, and all fields related to life sciences.</p> Use of Antenatal Services among Women in Farming Communities in Kogi State 2021-03-22T16:25:02+07:00 Muhammed K. Ibrahim Ezekiel Haruna Safiyat E. Tanko <p>This study examined the use of antenatal services among women in farming communities in Kogi State, Nigeria. A multi-stage sampling technique was employed to collect primary data from 150 randomly selected farmers through the use of structured questionnaire. The data was analysed using descriptive statistics and logit regression model. Analysis of socio-economic variable revealed that majority (73.4%) of the respondents were below the age 40 years, with a mean household size of 6 members. About 94.7% of the respondents used antenatal care services covering an average distance of 4km in getting to nearest health facility. The study also revealed that majority (92.7%) of the respondents were aware of healthcare facilities availability and they mostly got their information from family members and friends. The use of antenatal care services among women was high and majority (95.3%) of the respondents had the knowledge of where antenatal centre is located. Also majority of the respondents visited those centres in their last pregnancy with frequency of visit between 1- 4 times. Previous visits, nearness to ANC centres and formal education positively influence the use of ANC services among women in the study area. High cost of antenatal services and poor level of information were found to be the most significant challenges facing women access to antenatal care services. &nbsp;We recommend that government should subsidize health care services for women in order to improve their affordability.</p> 2021-02-23T00:00:00+07:00 Copyright (c) 2021 Journal La Lifesci Physical Mechanical Properties of Biodegradable Plastics from Cassava Starch with Variation of Bagasse and Glycerol. 2021-03-22T10:49:00+07:00 Triana Lindriati Andrew Setiawan Rusdianto Bustani Pakartiko Firda Ainia Adha <p><em>Environmental problems are related to plastic waste that difficult to degrade naturally. Polysaccharides in the form of gelatinized cassava starch and bagasse containing 52.70% cellulose can replace commercial plastic polymers. This research was aimed to determine the effect of variations in bagasse and glycerol on physical, mechanical and biodegradation tests. The study used 2 factors CRD consisting of the addition of bagasse and glycerol. The results showed that the addition of bagasse and glycerol reducing the brightness with a value range of 44.82-76.15 and increased absorption between 2.61-10.64%. The thickness occurred between 0.79-1.11mm. The tensile strength values are 8.30-14.33 MPa, inversely proportional to the elongation value between 11.85-94.22%, but directly proportional to the modulus young value between 0.09-1.24 MPa. The highest biodegradation values were 0% bagasse and 40% glycerol. The three best treatments were found in the combination treatment of bagasse and glycerol 0% 20%, 0% 30%, and 0% 40%.</em></p> 2021-02-23T13:16:24+07:00 Copyright (c) 2021 Journal La Lifesci Genome Editing of Saccharomyces Cerevisiae Using CRISPR-Cas9 System 2021-03-23T08:50:03+07:00 Yaseen Ismael Imran Ibrahim Abdulla Ahmed Ahmed Ali Mhawesh <p><em>Saccharomyces cerevisiae </em>is an important yeast has been exploited for a long time to produce alcohol or bread. Moreover, genetically engineered <em>S. cerevisiae</em> cells continue to be used as cell factories for production of biofuels, pharmaceutical proteins and food additives. Genetically modified strain of <em>S. cerevisiae</em> created using traditional methods is laborious and time consuming. Recently, originally an immune system in archaea and bacteria, Clustered regularly interspaced short palindromic repeats “CRISPR” and CRISPR-associated “Cas” have been used exploited&nbsp; as a flexible tool for genome editing. Until now, this tool has been applied to many organisms including yeast. Here, we review the importance of <em>S. cerevisiae</em> as an industrial platform and the use of CRISPR/Cas system and its applications in research and industry of this yeast.</p> <p>&nbsp;</p> 2021-03-23T08:50:03+07:00 Copyright (c) 2021 Journal La Lifesci Effect Jamblang Stem Barks, Mangosteen Peel, and Cinnamon to Blood Sugar Levels 2021-04-19T09:50:20+07:00 Thabed Tholib Baladraf <p>Diabetes mellitus ranks seventh as the most deadly disease with a prevalence of 1.9% and is dominated by type 2 diabetes mellitus with 95%. A solution is needed to overcome this problem, one of which is by utilizing jamblang stem barks, cinnamon, and mangosteen peel which are packaged in a popular product, namely steeping tea bags. This research aimed to determine the effect and potential biodiversity of extracts of jamblang stem barks, cinnamon and mangosteen peel as an antidiabetic drug in the form of infusion in rats induced by alloxan. This research used one-factor completely randomized design (CRD) with a linear model. Data were analyzed by One Way ANOVA and Post-hoc Tukey. The sample used was male mice aged 2-3 months with a body weight of ± 20 grams. The sample consisted of 25 individuals and divided into 5 groups. group I (control), group II (alloxan induction + jamblang stem barks extract, cinnamon, and mangosteen peel 1.35 grams / 200gBB), group III (alloxan induction + mangosteen peel 1.35 grams / 200gBB), group IV (alloxan induction + wood sweet 1.35 gram / 200gBB), group V (alloxan induction + jamblang stem barks 1.35 gram / 200gBB). Day 21, the amount of increase in blood sugar levels was observed and assessed based on the results of the tools used. The results showed that the infusion of jamblang stem barks, cinnamon and mangosteen peel had a significant effect on reducing blood sugar so that it could prevent type 2 diabetes mellitus.</p> 2021-04-12T13:28:47+07:00 Copyright (c) 2021 Journal La Lifesci Influence of Habitat Structure and Ecological Zones on Amphibian Diversity in Rivers State, Nigeria 2021-04-30T09:52:11+07:00 Ekerette I B Robert B Nwachukwu K Laku C B Amuzie C C <p><em>This study investigates the influence of habitat structure on the species diversity of anurans of Rivers State, Nigeria.&nbsp; This research was conducted between 2015 and 2019. The survey covered seven Local Government Areas (LGAs) and three ecological zones (lowland forest, freshwater swamp and short mangrove). The habitats included farmlands, fallow areas, swamps, streams and inundated areas, bush paths, human settlements and forested areas. Visual encounter and acoustic survey methods were used and species identification was aided by appropriate taxonomical keys. Diversity indices were computed using Past-exe statistical software. Nineteen amphibian species were encountered including members of the Arthroleptidae, Bufonidae, Dicroglossidae, Ranidae, Hyperoliidae, Ptychadenidae and Pipidae. Freshwater swamps and short mangrove ecological zones (characterized by human settlements and farmlands) had the lowest diversity of two and five amphibian species, respectively. The highest diversity comprised of eighteen amphibian species was recorded in forested habitats of the lowland forest/freshwater swamp ecological zone. 569 individuals were encountered at lowland forest/freshwater swamp ecological zone; 215 at lowland forest; 60 at short mangrove; and 28 at freshwater swamp ecological zone. Shannon diversity was highest in the lowland/freshwater swamp ecological zone (H=2.12) and lowest in the freshwater swamp zone (H=0.52). The results show that forested habitats comprised of the combination of lowland forest and freshwater swamp ecosystems host a greater diversity of amphibian species. We therefore recommend that lowland and freshwater swamp ecosystems in the State should be protected to support greater diversity of amphibians.</em></p> 2021-04-19T09:49:39+07:00 Copyright (c) 2021 Journal La Lifesci