Journal La Lifesci https://newinera.com/index.php/JournalLaLifesci <p>International <strong>Journal La </strong><strong>Lifesci</strong>&nbsp; ISSN 2721-1207 (online) and ISSN 2721-1304 (Print) includes all the areas of research activities in all fields of Life Sciences such as Agricultural, Fisheries, Earth, Environmental Science, Botany, Zoology, Microbiology, Ecology, Ethnobiology, Biodiversity And Conservation Biology, Genetics, Biochemistry , Bioinformatics, Biophysics, Biostatistics, Cytobiology Developmental Biology, Entomology, Immunology , Molecular Biology, Virology, agronomy, plant and animal breeding, agricultural economics and rural sociology,&nbsp; Veterinary science, Ornithology, Primatology, Biogeography, Histology, Marine biology, Biochemical Sciences Aquaculture, Fishery Hydrography, Fishery Engineering, Aquatic ecosystem, Fish farming, Fisheries management, Fishery Biology, Wild fisheries, Ocean fisheries, and all fields related to life sciences.</p> Newinera Publisher en-US Journal La Lifesci 2721-1304 Income Analiysis of Langsat Farmers https://newinera.com/index.php/JournalLaLifesci/article/view/3122 <p><em>The purpose of this study was to analyze the income of farmers who grow langsat in Sulewatang village and evaluate the feasibility of their agricultural activities using profit ratio (R/C) analysis. The primary data used were obtained from direct interviews with farmers who grow langsat, as well as secondary data collected from related institutions. The analysis method includes calculating total income (TR = P × Q), total costs (TC = FC + VC), and profit ratio (R/C = TR ÷ TC). The results showed that the average income of farmers who grow langsat reached 2,241,190 Indonesian rupiah, with a total production cost of 360,769 Indonesian rupiah. Thus, the farmer's net income was 1,880,421 Indonesian rupiah. The profitability coefficient (R/C) value of 6.21 indicates that R/C &gt; 1, which means that langsat cultivation in Sulewatang village is profitable and feasible to be further developed.</em></p> Yerike Yerike Jumriani Dambe Hamsah Hamsah Copyright (c) 2026 Journal La Lifesci http://creativecommons.org/licenses/by-sa/4.0/ 2026-03-30 2026-03-30 7 2 104 116 10.37899/journallalifesci.v7i2.3122 Alterations in VKORC1 Gene and Cardiac Enzymes in Acute and Chronic Congestive Heart Failure https://newinera.com/index.php/JournalLaLifesci/article/view/3136 <p><em>disorder that activates compensatory mechanisms, including neurohormonal responses and circulatory adjustments, which initially preserve perfusion but later contribute to systemic and pulmonary congestion. This study examined the effect of acute and chronic CHF on VKORC1 gene overexpression and measured serum cardiac enzymes, namely troponin I (cTn I), creatine kinase MB (CK MB), lactate dehydrogenase (L LDH), and myoglobin (MYO MB), at each disease phase. The study included 40 CHF patients, consisting of 20 with acute CHF (ACHF) characterized by severe sudden cardiac symptoms and 20 with chronic CHF (CCHF) showing gradual progression, along with 20 healthy participants as a negative control group. Venous blood samples were collected for molecular analysis using quantitative PCR and for serological assessment of cardiac enzymes using quantitative ELISA. Statistical significance was determined using GraphPad Prism at p &lt; 0.05. The results showed that, across all groups, the GG genotype frequency was significantly higher than GA and AA. However, allele frequency analysis of VKORC1 (-1639G&gt;A) rs9923231 revealed significantly increased GA and AA frequencies in both ACHF and CCHF patients. Serologically, cTn I, CK MB, L LDH, and MYO MB levels were significantly elevated in both patient groups compared with controls. Between ACHF and CCHF, only CK MB was significantly higher in CCHF, while cTn I, L LDH, and MYO MB showed no significant difference. This appears to be the first Iraqi study linking VKORC1 with CHF phases, suggesting its potential as an indicator of both acute and chronic CHF, although further investigation is needed</em></p> Dhuha Abdullah Abbas Copyright (c) 2026 Journal La Lifesci http://creativecommons.org/licenses/by-sa/4.0/ 2026-04-11 2026-04-11 7 2 117 131 10.37899/journallalifesci.v7i2.3136 Installation Performance Evaluation Domestic Wastewater Treatment Plant in Fulfil Minister of Environment and Forestry Regulation No. 11 of 2025 https://newinera.com/index.php/JournalLaLifesci/article/view/3144 <p><em>Wastewater management domestic is aspect crucial in control pollution environment. especially in developing areas like Merauke Regency. Research This aim evaluate performance Installation Domestic Wastewater Treatment Plant ( WWTP) in Housing District Ulilin in fulfil standard quality Minister of Environment and Forestry Regulation No. 11 of 2025. Research method use approach quantitative descriptive – evaluative with design studies case. Taking sample carried out at the inlet and outlet of the IPAL during September–October 2025 using grab sampling method. with Analysis of TSS, pH, BOD₅, COD, oil and fat, NH₃-N, and total coliform parameters based on Standard Methods (APHA, 2017). The results show that characteristics influence majority exceed standard quality. especially in the parameters BOD₅ (33.4 mg/L), COD (122.5 mg/L), TSS (35.5 mg/L), oil and fat (6.15 mg/L), NH₃-N (13.1 mg/L), and total coliform (3.59×10³ MPN/100 mL). After processing. all effluent parameters fulfil standard quality with level 100% compliance Efficiency elimination show very good performance. with decline significant on BOD₅ (83.38%), COD (84.04%), TSS (49.30%), and NH₃-N (93.13%). High safety margin to standard quality show system own capacity adequate and resilient reserves to fluctuations burden polluters. In overall. domestic wastewater treatment plant District Ulilin operate effective. stable. and feasible in support protection quality environment in a way sustainabl.</em></p> Irfan Syarifandi Natsar Desi Muhammad Chaerul Erniati Erniati A.M.Yusuf Harun Mahmud Mahmud Copyright (c) 2026 Journal La Lifesci http://creativecommons.org/licenses/by-sa/4.0/ 2026-05-11 2026-05-11 7 2 132 141 10.37899/journallalifesci.v7i2.3144 The Influence of Sociodemographic Factors on Food Waste Creation https://newinera.com/index.php/JournalLaLifesci/article/view/3145 <p><em>Food waste has emerged as a global issue of growing international concern, with developing countries striving to identify effective strategies to address the problem of food-related waste. In this study, food waste refers specifically to edible household food residues that are discarded despite being fit for consumption. This research aims to analyze the generation of food waste in Singkawang City and to examine the relationship between sociodemographic factors and the amount of food waste generated. The household sampling technique employed in this study was proportionate stratified random sampling, while the selection of individual family member respondents within each household was conducted using incidental sampling. The analytical methods applied include descriptive analysis, food waste quantification, and multiple linear regression analysis. The findings indicate that the highest level of food waste generation occurred in Singkawang Tengah District, producing the largest volume of waste (28,370 kg/year), while the lowest generation was recorded in Singkawang Utara District (7,140 kg/year). Furthermore, the results of the analysis examining the relationship between sociodemographic factors and food waste generation demonstrate that, simultaneously, sociodemographic variables (age, gender, income, education level, occupation, and number of household members) significantly influence the dependent variable (food waste generation). Partially, however, age, gender, education level, and occupation were found not to have a significant effect on food waste generation.</em></p> Raisa Maharani Maswadi Maswadi Dewi Kurniati Copyright (c) 2026 Journal La Lifesci http://creativecommons.org/licenses/by-sa/4.0/ 2026-05-11 2026-05-11 7 2 142 152 10.37899/journallalifesci.v7i2.3145 The Relationship Between CRP Level and Age in Salmonella Typhi Patient https://newinera.com/index.php/JournalLaLifesci/article/view/3207 <p><em>Typhoid fever has increased in some populations, hence the current study, aimed to evaluate the prevalence of salmonella seropositivity and other relevant factors such as sex, age, and CRP positivity among febrile patients, especially among the patients estimated to be suffering with typhoid fever located at Al-Najaf province in Iraq. The study comprised 79 febrile patients who presented for intestinal complaints at Al-Hakeem General Hospital, AL Sajad Hospital, and AL Najaf Hospital’s serological departments within Al-Najaf province between October 2024 and January 2025. Those were between 1 and 75 years of age. This study employed rapid testing modalities for the qualitative detection of typhoid infection and assessment of serum CRP levels. Among the 79 febrile patients screened, 41 (51.9%) were female, 38 male (48.1%) was assessed. On typhoid seropositivity, 23 individuals (29.2%) tested positive, and 56 (70.8%) negative, but no differences between gender were found (P=0.36). Age distribution of febrile patients was further classified into 5 groups, 1-15 years, 16-30 years, 31-45 years, 46-60 years, 61-75 years. Notably, the greatest prevalence occurred at a range of 1 to 15 years of age (27.8%), then in the 16-30 year group (25.3%). Among those diagnosed as positive for Salmonella seropositivity, it was reported that those ages 61-75 had a higher positivity rate of 12.7%, indicating a statistically significant difference (P=0.001).</em></p> Haider Abdul-Moneem Al-Mothafer Copyright (c) 2026 Journal La Lifesci http://creativecommons.org/licenses/by-sa/4.0/ 2026-05-11 2026-05-11 7 2 153 165 10.37899/journallalifesci.v7i2.3207