Serological Detection of Toxoplasma Gondii in Animals and Human in Baghdad Province, Iraq

Ali A. Shatti

doi:10.37899/journallalifesci.v5i2.1262

Ali A. Shatti Department of Microbiology, College of Veterinary Medicine, University of Wasit, Wasit, Iraq

DOI: https://doi.org/10.37899/journallalifesci.v5i2.1262

Keywords: Zoonotic diseases, ELISA, Floatation, Cat, Sheep

Abstract

This study aims to investigate the prevalence of Toxoplasma gondii in field animals (cattle and sheep) and their owners at various areas in Baghdad province (Iraq) during September (2023) and February (2024). Also, it aims to identify the role of cat in transmission of infection to humans and animals. A total of 222 venous blood samples including 84 humans and 138 animals were collected under aseptic conditions into free-anticoagulant glass tubes. In addition, a total of 112 fecal cat samples were collected from different areas in Baghdad city into plastic containers to be examined by the flotation method. The results showed that 84 blood samples from animal owners detected the infection rate were 36.90%. In addition, there is a relationship between infection and age. The study shows that the highest age for infection is 26-30 years, and the percentage was 66.66%. also, the study included examining 138 blood samples from animals divided into 68 sheep and 70 cows’ infection parasite rate was also 41.30%, and through examining the collection of 120 samples of soil from different areas in the city of Baghdad, the percentage of infection with T. gondii parasite reached 32.50%, addition to the infection rate in cats was 50% by examining 112 stool samples collected from different areas in the same city. This study reported the increasing seroprevalence of T. gondii using different samples. The reason for this increase may be due to poor health conditions, the large number of stray cats, the accumulation of dirt, the low degree of health education, and ignorance of the sources of infection for the disease and the attempt to avoid them, as it was noted through the study that a large number of women raise cats in their homes. They do not know that cats are the source of the disease.

References

Abdulla, B. A. (2001). Toxoplasmosis in high risk pregnancies in Mosul. Iraq. Raf. J. Sci, 12(2), 1-4.

Abdullah, B. A., & Al-Ubbyde, G. T. (2012). Toxoplasmosis in high risk pregnant women and it's relation with some serological parameters in Nineveh governorate. Iraqi Journal of Veterinary Sciences, 26, 283-288. https://doi.org/10.33899/ijvs.2012.168738

Al-Bayati, L. H., Razooqi, M. A., & Saleem, H. D. (2021). Serological detection of Coxiella burnetii in raw milk of goats in Baghdad, Iraq. Biochemical & Cellular Archives, 21(2).

Al-Kappany, Y. M., Abbas, I. E., Devleesschauwer, B., Dorny, P., Jennes, M., & Cox, E. (2018). Seroprevalence of anti-Toxoplasma gondii antibodies in Egyptian sheep and goats. BMC veterinary research, 14, 1-5.

Alobaidy, H., Saleem, H. D., & Al-Dhalimy, A. M. B. (2022). Comparative physiological aspects of plasma hemostasis of some commercial fish species. Journal of Water and Land Development, (54).

Al-Sanjary, R. A., & Hussein, T. H. (2012). Using species-specific PCR technique to detect Toxoplasma gondii in broiler chickens. Iraqi Journal of Veterinary Sciences, 26(2), 53-56.

Fatohi, F. A. M. (1985). Detection of toxoplasmosis among different groups of populations in Mousl city by using IFAT and CFT (Doctoral dissertation, M. Sc. Thesis, College of Medicine, University of Mousl).

Gharban, H. A., Al-Ghuraibawi, H. N., Al-Rubaye, Z. A., Jahlol, H. A., Al-Zergany, A. A., & Al-Abedi, G. J. (2023). Most Clinically Detected Viral Diseases in Field Animals of Wasit Province, Iraq. Annals of the Romanian Society for Cell Biology, 27(01), 154-168.

Guo, M., Mishra, A., Buchanan, R. L., Dubey, J. P., Hill, D. E., Gamble, H. R., & Pradhan, A. K. (2016). A systematic meta-analysis of Toxoplasma gondii prevalence in food animals in the United States. Foodborne pathogens and disease, 13(3), 109-118.

Jawetz, M. (2007). Adelberg's Medical Microbiology. Antibacterial and Antifungal chemotherapy (Prentice-Hall International Inc).

Madjeed Haddao, K., Dawood Saleem, H., Hameed, N. M., Mahdi Rheima, A., Alkhafaje, W. K., Salaam Abood, E., & Balasim Al-Dahy, L. (2022). Investigation of in vitro Cytotoxicity of Chelidonium majus against Leishmania Major. Archives of Razi Institute, 77(3), 1211-1214.

Majeed, B. A., & Abbas, W. H. (2018). Serological and molecular detection of Toxoplasma gondii in meat and minced meat in Basra City. Basrah Journal of Veterinary Research, 17(3).

Mohammed, L. J., and Al-Janabi, M. S. (2019). Seroprevalence of toxoplasmosis in aborted women in Babylon Province, Iraq. Medical Journal of Babylon, 16(3), 188-191.

Noori, A. (2021). Comparisons of Toxoplasma gondii Prevalence in Rural and Urban Areas of Al-Najaf Province of Iraq Using Serological Methods. Archives of Razi Institute, 76(6), 1695. https://doi.org/10.22092/ari.2021.356315.1822

Peretz, D. (1994). The Middle East Today. Bloomsbury Publishing USA.

Rufash, H. N., & Yousif, J. J. (2018). Effect of genotypes of Toxoplasma gondii parasite on the level of some cytokines and markers in aborted women in Najaf governorate. Biochemical and Cellular Archives, 18(1).

Saleem, H. D., & Al-Samarai, F. R. (2018). Plasma superoxide dismutase, malondialdehyde and lymphocyte DNA damage in horses with Theileria equi detected by qPCR. Online Journal of Veterinary Research, 22(6), 457-466.

Saleem, H. D., Al-Obaidi, A. H., & Al-Tmemy, W. B. (2021). Histopathological Changes Due To Toxic Effect Of Aflatoxin B1 On Liver, Kidney And Therapeutic/Preventive Role Of Camel Milk. Biochemical and Cellular Archives, 21(1).

Saleem, H. D., Hamza, T. A., Izzat, S. E., Hamad, D. A., Abdulhasan, M. J., & Adhab, A. H. (2022). Role Silver and Bimetallic Nano Particles Synthesized by Green Chemical Methods for their Therapeutic Potential for Cancer: A Review. Journal of Pharmaceutical Quality Assurance, 13(2), 222-226.

Salih, J. M., Mero, W. M. M., & Eassa, S. (2020). Seroprevalence and some demographic factors associated with Toxoplasma gondii infection among male population in Duhok Province/Iraq. Baghdad Science Journal, 17(2), 0431-0431.

Shallal Shahatha, S. (2017). Prevalence Of Toxoplasma Gondii Parasite And Their Causes In Al-Ramadi City Al-Anbar Governorate. Iraqi Journal of Desert Studies, 7(2), 109-116.

Tonouhewa, A. B. N., Akpo, Y., Sessou, P., Adoligbe, C., Yessinou, E., Hounmanou, Y. G., & Farougou, S. (2017). Toxoplasma gondii infection in meat animals from Africa: systematic review and meta-analysis of sero-epidemiological studies. Veterinary World, 10(2), 194.

Wallander, C. (2016). Toxoplasma gondii in wild boars and domestic pigs in Sweden. Acta Universitatis Agriculturae Sueciae, (2016: 12).

Wassef, R., & Abdel-Malek, R. (2019). Validity of a new immunochromatographic test in detection of Toxoplasma gondii in cancer patients. Journal of parasitic diseases, 43, 83-86.

Wassef, R., and Abdel-Malek, R. (2019). Validity of a new immunochromatographic test in detection of Toxoplasma gondii in cancer patients. Journal of parasitic diseases, 43, 83-86.

Wilson, W., Taubert, K. A., Gewitz, M., Lockhart, P. B., Baddour, L. M., Levison, M., & Durack, D. T. (2017). Prevention of infective endocarditis: guidelines from the American heart association: a guideline from the American heart association rheumatic fever, endocarditis, and Kawasaki disease committee, council on cardiovascular disease in the young, and the council on clinical cardiology, council on cardiovascular surgery and anesthesia, and the quality of care and outcomes research interdisciplinary working group. Circulation, 116(15), 1736-1754. https://doi.org/10.1161/CIRCULATIONAHA.106.183095