Establishment of Hematological Reference intervals for healthy Children in Elobied City, Sudan

Tarig Osman Khalafallah  Ahmed; Malaz Elfatih Abd-elkareem  Ahmed; Ream Elzain  Abdelgadir; Hiba Awadelkareem Osman  Fadl; Assad MA.  Babker

doi:10.37899/journallamedihealtico.v2i4.382

Tarig Osman Khalafallah Ahmed Faculty of Medical Laboratory Science, Department of Hematology, University of Kordofan, El-Obeid, Sudan
Malaz Elfatih Abd-elkareem Ahmed Department of Hematology, Elobeid Teaching Hospital, Ministry of Health, El-Obeid, Sudan
Ream Elzain Abdelgadir Faculty of Medical Laboratory Science, Department of Hematology, University of Kordofan, El-Obeid, Sudan
Hiba Awadelkareem Osman Fadl Department of Hematology, Faculty of Medical Laboratory Sciences, AL-Neelain University and Medical Laboratory Department, Sudanese Medical Research Association (SMRA), Khartoum, Sudan
Assad MA. Babker Department of Medical Laboratory Sciences, College of Health Sciences, Gulf Medical University, Ajman, United Arab Emirates

DOI: https://doi.org/10.37899/journallamedihealtico.v2i4.382

Keywords: Reference Intervals, Hemogram, Hb, Healthy Children, Elobied-Sudan

Abstract

Hemogram Reference intervals are established since a healthy population is critical to accurately interpret laboratory tests, which include Hemoglobin estimation, Red blood cells count and indices, White blood cells count and differential in addition to Platelets count. This study aims to establish the reference interval of the complete hemogram amongst healthy Sudanese children in Elobied city, Sudan. A descriptive cross-sectional study included 354 healthy children, aged between 3 to 17 years, who were categorized into three groups according to age. A questionnaire was fulfilled, EDTA anti-coagulated venous blood sample was collected from each child then the complete hemogram was performed automated hematological analyzer (Sysmex Xp 300), Finally, Data was analyzed by a software program (SPSS version 21). The hematological reference intervals for healthy children [Hb g/dl, HCT%, RBCs count x106μL, MCV/fL ,MCH /pg., MCHC g/dl ,WBCs count x10³/μL, Neutrophil count%, Eosinophil count %, Basophile count %, lymphocyte count ,Monocytes count%, RDW CV and PLTs count×10³/μL] are [(12.4±1.2),(37±4),(5.0 ±0.4), (82±5.0), (26±3.0), (32±3.0) ,(7.0±2.0) (47±10), (1±0.1) ,(0±0),(45± 10),(7 ± 4), (13±1.8) &(227±91)] respectively. The hematological RI for healthy children in Elobied was established in this study to be representative of this population, there was a significant gender-based difference in all the evaluated hematological parameters, they were found to be higher in males than in females except for basophil (%). Finally, the results of this study would shed a light on the importance of establishing RI for the children population in Elobied.

References

Agabeldour, A. A., Khalafallah, T. O., & AbdAllah, A. M. (2015). Hematological changes among Sudanese petroleum workers with a broad range of benzene exposure: Sch. J. App. Med. Sci, 3(8D), 3054-3056.

Akunov, A., Sydykov, A., Toktash, T., Doolotova, A., & Sarybaev, A. (2018). Hemoglobin changes after long-term intermittent work at high altitude. Frontiers in physiology, 9, 1552.

Al-Jafar, H. (2016). Provisional Kuwaiti children hematology reference range. J Hematol Thromb, 2(2), 1-6.

Bain, B. J. (1985). Platelet count and platelet size in males and females. Scandinavian journal of haematology, 35(1), 77-79.

Balduini, C. L., & Noris, P. (2014). Platelet count and aging. Haematologica, 99(6), 953.

Béavogui, A. H., Touré, A. A., Camara, D., Doumbouya, A., Sylla, M. M., Touré, S., ... & Djimdé, A. (2020). Haematological and Biochemical Reference Values for Healthy Population of Maferinyah Rural Community in Guinea. BioMed research international, 2020.

Bruce, B. David, M. & Atul, B. (2002). “Normal ranges,” in Blood cells— A Practical Guide, Blackwell scientific Publications, Oxford, UK, 3th edition,

Buchanan, A. M., Muro, F. J., Gratz, J., Crump, J. A., Musyoka, A. M., Sichangi, M. W., ... & Cunningham, C. K. (2010). Establishment of haematological and immunological reference values for healthy Tanzanian children in Kilimanjaro Region. Tropical Medicine & International Health, 15(9), 1011-1021.

Costanzo, L. S. (2007). Physiology Hagerstwon. MD:(Ed) Lippincott Williams and Wilkins.

Dacie, J. V., & Lewis, S. M. (2006). Practical Haematology 10th Edition Churchill Livingstone. Edinburgh, London, 398-440.

Gomella L, Leonard G and steven A. clinicians pocket reference 11th ed new York 31, (2013).

Gomez, P., Coca, C., Vargas, C., Acebillo, J., & Martinez, A. (1984). Normal reference-intervals for 20 biochemical variables in healthy infants, children, and adolescents. Clinical chemistry, 30(3), 407-412.

Hoffbrand, A. V., Peltit, J. E., & Moss, P. (2003). Essential hematology. haemostasis, 16, 17.

Jung, B., & Adeli, K. (2009). Clinical laboratory reference intervals in pediatrics: the CALIPER initiative. Clinical biochemistry, 42(16-17), 1589-1595.

Kabir L, Akpa B, Nwagu B, Adeyinka C, and Bello K. ,(2011) Hematological reference values for adolescent and young adults in a rural population in northern America, tropica Med intsci: 16(9):204- 211.

Karita, E., Ketter, N., Price, M. A., Kayitenkore, K., Kaleebu, P., Nanvubya, A., ... & Kamali, A. (2009). CLSI-derived hematology and biochemistry reference intervals for healthy adults in eastern and southern Africa. PloS one, 4(2), e4401.

Lugada, E. S., Mermin, J., Kaharuza, F., Ulvestad, E., Were, W., Langeland, N., ... & Downing, R. (2004). Population-based hematologic and immunologic reference values for a healthy Ugandan population. Clinical and Vaccine Immunology, 11(1), 29-34.

Mathur S, Schexneider K & Hutchison R. (2011). Hematopoiesis In: Henry’s clinical Diagnosis. 8th edition, Cecil medicine. (UK).524- 531.

Mohamed, A. O., Hamza, K. M., & Babker, A. M. (2016). Physiological changes in some hematological and coagulation profile among Sudanese healthy pregnant women. Int J Med Sci Public Health, 5(3), 525-8.

Quintó, L., Aponte, J. J., Sacarlal, J., Espasa, M., Aide, P., Mandomando, I., ... & Alonso, P. L. (2006). Haematological and biochemical indices in young African children: in search of reference intervals. Tropical Medicine & International Health, 11(11), 1741-1748.

Saathoff, E., Schneider, P., Kleinfeldt, V., Geis, S., Haule, D., Maboko, L., ... & Hoelscher, M. (2008). Laboratory reference values for healthy adults from southern Tanzania. Tropical Medicine & International Health, 13(5), 612-625.

Sahoo, D., Gosaib, H., Harsodaa, J. M., & Palana, B. M. (2015). A comparative hematological profile study among young individuals. Canadian Journal of Basic and Applied Sciences, 3, 178-81.

Segal, J. B., & Moliterno, A. R. (2006). Platelet counts differ by sex, ethnicity, and age in the United States. Annals of epidemiology, 16(2), 123-130.

Shaw, J. L., Binesh Marvasti, T., Colantonio, D., & Adeli, K. (2013). Pediatric reference intervals: challenges and recent initiatives. Critical reviews in clinical laboratory sciences, 50(2), 37-50.

Tonbul, A., Tayman, C., Catal, F., Kara, S., & Tatli, M. M. (2011). Red cell distribution width (RDW) in the newborn: normative data. Journal of clinical laboratory analysis, 25(6), 422-425.

Zeh, C., Amornkul, P. N., Inzaule, S., Ondoa, P., Oyaro, B., Mwaengo, D. M., ... & Laserson, K. (2011). Population-based biochemistry, immunologic and hematological reference values for adolescents and young adults in a rural population in Western Kenya. PLOS one, 6(6), e21040.