Immunomodulatory Effects of Ethanol Extract of Lime Peel on Phagocytosis Activity and Delayed-Type Hypersensitivity Response
Abstract
The natural human environment is inhabited by various organisms, including those harmful to the body such as bacteria, viruses, fungi, and other pathogens. This condition weakens the immune system's response to pathogens, making the body more susceptible to diseases such as rheumatoid arthritis, cancer, and other inflammations. In some cases, this condition can be fatal, leading to death. This study aims to examine the immunomodulatory effects of Ethanol Extract of Lime Peel on phagocytosis activity and delayed-type hypersensitivity response in experimental animals. The research methods include sample collection and processing, plant identification, extract preparation, and immunomodulatory effect testing. Phytochemical screening results showed the presence of alkaloids, glycosides, flavonoids, tannins, and steroids in the extract of the lime peel. Simplicia and ethanol extract of lime peel characterization yielded results meet the standards of the Indonesian Herbal Pharmacopoeia 2nd edition. Immunomodulatory effect testing was conducted through phagocytosis activity and delayed-type hypersensitivity response assays. The results showed that the extract at doses of 25, 50, and 100 mg/kgBW had immunostimulatory effects in enhancing phagocytosis activity, while doses of 17.5 mg/kgBW, 35 mg/kgBW, and 70 mg/kgBW significantly increased delayed-type hypersensitivity response compared to the negative control. These findings indicate the potential of ethanol extract of Citrus aurantifolia as an immunomodulator that can enhance the body's immune system. This study provides an important contribution to the development of immunomodulatory therapy derived from natural ingredients such as lime peel for managing immunity-related diseases with lower side effects.
References
Asfianti, V., Sapitri, A., & Marbun, E. D. (2022). The Immunomodulatory Activity of Ethanol Extract of Attarasa Bark and Fruit (Litsea Cubeba (lour.) Pers.) Toward Carbon Clearance of Mice (Mus Musculus). Jurnal Ilmiah Farmasi, 175–183. https://doi.org/10.20885/jif.specialissue2022.art20
Azwari, Z., Juwita, D. A., Susanty, A., Putri, N., Hefni, D., Dachriyanus, & Wahyuni, F. S. (2021). The Immunomodulatory Activities of Alkaloid (Vf-1) Isolated from Stem Bark of Tampa Badak ( Voacanga Foetida (Bl) Rolfe) on Raw 264.7 Cells. Proceedings of the 2nd International Conference on Contemporary Science and Clinical Pharmacy 2021 (ICCSCP 2021), 268–276. https://doi.org/10.2991/ahsr.k.211105.039
Behl, T., Kumar, K., Brisc, C., Rus, M., Nistor-Cseppento, D. C., Bustea, C., Aron, R. A. C., Pantis, C., Zengin, G., Sehgal, A., Kaur, R., Kumar, A., Arora, S., Setia, D., Chandel, D., & Bungau, S. (2021). Exploring the Multifocal Role of Phytochemicals as Immunomodulators. Biomedicine & Pharmacotherapy, 133, 110959. https://doi.org/10.1016/j.biopha.2020.110959
Bondonno, N. P., Lewis, J. R., Blekkenhorst, L. C., Bondonno, C. P., Shin, J. HC., Croft, K. D., Woodman, R. J., Wong, G., Lim, W. H., Gopinath, B., Flood, V. M., Russell, J., Mitchell, P., & Hodgson, J. M. (2020). Association of Flavonoids and Flavonoid-Rich Foods with All-Cause Mortality: The Blue Mountains Eye Study. Clinical Nutrition, 39(1), 141–150. https://doi.org/10.1016/j.clnu.2019.01.004
Borowicz, P., Chan, H., Hauge, A., & Spurkland, A. (2020). Adaptor Proteins: Flexible and Dynamic Modulators of Immune Cell Signalling. Scandinavian Journal of Immunology, 92(5), 1–26. https://doi.org/10.1111/sji.12951
Calder, P. C. (2021). Nutrition and Immunity: Lessons for COVID-19. Nutrition & Diabetes, 11(1), 1–18. https://doi.org/10.1038/s41387-021-00165-0
Chen, Wang, Tan, Hu, Sundararajan, & Zhou. (2020). Profiling of Flavonoid and Antioxidant Activity of Fruit Tissues from 27 Chinese Local Citrus Cultivars. Plants, 9(2), 1–18. https://doi.org/10.3390/plants9020196
Denaro, M., Smeriglio, A., & Trombetta, D. (2021). Antioxidant and Anti-Inflammatory Activity of Citrus Flavanones Mix and Its Stability after In Vitro Simulated Digestion. Antioxidants, 10(2), 1–17. https://doi.org/10.3390/antiox10020140
Dogan, E. (2022). Levamizol Uygulanmasının Şarbon Hastalığına Karşı Aşılanmış Sığırlarda Bağışıklık Sistemi Üzerine Etkileri. Kocatepe Veterinary Journal, 15(3), 348–354. https://doi.org/10.30607/kvj.1082258
Erjon, E. (2022). Efek Imunostimulan Ekstrak Etanol Daun Jengkol (Archidendron Jiringan (Jack) I.C. Nielsen) Pada Mencit Putih Jantan. Jurnal Ilmiah Farmasi Farmasyifa, 5(1), 62–70. https://doi.org/10.29313/jiff.v5i1.7704
Fristiohady, A., Wahyuni, W., Malik, F., Leorita, M., Yusuf, M. I., Febriansyah, H., & Sahidin, S. (2019). Efek Imunomodulator Ekstrak Etanol Spons Xestospongia Sp. Terhadap Aktivitas Fagositosis Makrofag Pada Mencit Jantan Galur Balb/C. Jurnal Mandala Pharmacon Indonesia, 5(01), 15–30. https://doi.org/10.35311/jmpi.v5i01.38
Khuluq, H., Cahyani, T., Kurniawan, I., Hemas, E., Agustina, N., & Agustin, S. T. (2021). Herbal Medicine For Immunostimulant In Kebumen Districts: An Ethnobotany Study. Urecol Journal. Part C: Health Sciences, 1(2), 38–45. https://doi.org/10.53017/ujhs.74
Kirana, I. D. A., Bodhi, W., Sari Lebang, J., & Fatimawali, F. (2023). Uji Aktivitas Fagositosis Makrofag Dari Ektrak Kulit Buah Jeruk Nipis (Citrus Aurantifolia) Sebagai Imunomodulator. Jurnal Kesehatan Tambusai, 4(3), 3021–3027. https://doi.org/10.31004/JKT.V4I3.16830
Kotala, S., & Kurnia, T. S. (2022). Eksplorasi Tumbuhan Obat Berpotensi Imunomodulator Di Kecamatan Leihitu Kabupaten Maluku Tengah. Sainmatika: Jurnal Ilmiah Matematika Dan Ilmu Pengetahuan Alam, 19(2), 186–200. https://doi.org/10.31851/sainmatika.v19i2.9508
Lu, Q., Xu, J., Jiang, H., Wei, Q., Huang, R., & Huang, G. (2022). The Bone-Protective Mechanisms of Active Components from TCM Drugs in Rheumatoid Arthritis Treatment. Frontiers in Pharmacology, 13, 1–15. https://doi.org/10.3389/fphar.2022.1000865
Luhurningtyas, F. P., Dyahariesti, N., & M, S. F. E. (2020). Uji Efek Imunomodulator Ekstrak Biji Karika (Carica pubescens Lenne K. Koch) terhadap Peningkatan Aktivitas Fagositosis pada Mencit Putih Swiss Webster. Pharmaceutical and Biomedical Sciences Journal (PBSJ), 2(1), 27–34. https://doi.org/10.15408/pbsj.v2i1.14436
Malik, F., Bafadal, M., Munasari, D., Andriani, R., Yusuf, Muh. I., Sahidin, S., Wahyuni, W., Fristiohady, A., & Nurfinti, W. O. (2022). Aktivitas Imunomodulator Ekstrak Etanol Buah Etlingera rubroloba A.D. Poulsen Terhadap Fagositosis Sel Makrofag Pada Tikus Jantan Galur Wistar. Jurnal Mandala Pharmacon Indonesia, 8(1), 96–112. https://doi.org/10.35311/jmpi.v8i1.171
Masniah, M., Rezi, J., & Faisal, A. P. (2021). Isolasi Senyawa Aktif dan Uji Aktivitas Ekstrak Jahe Merah (Zingiber Officinale) Sebagai Imunomodulator. Jurnal Riset Kefarmasian Indonesia, 3(2), 77–91. https://doi.org/10.33759/jrki.v3i2.131
Phucharoenrak, P., Muangnoi, C., & Trachootham, D. (2023). Metabolomic Analysis of Phytochemical Compounds from Ethanolic Extract of Lime (Citrus aurantifolia) Peel and Its Anti-Cancer Effects against Human Hepatocellular Carcinoma Cells. Molecules, 28(7), 2965. https://doi.org/10.3390/molecules28072965
Putri Prakarsa, C., Bodhi, W., & Datu, O. S. (2024). Uji Aktivitas Fagositosis Makrofaag Ekstrak Tanaman Gedi Abelmoschus Manihot (L) Sebagai Imunomodulator . PHARMACON, 13(1), 419–430. https://doi.org/10.35799/PHA.13.2024.49698
Sari, W. Y., Yuliastuti, D., & Ramadhan, M. F. (2021). Uji Imunomodulator Sirup Ekstrak Kulit Jeruk Nipis Citrus Aurantiifolia. Jurnal Farmasetis, 10(2), 145–150. https://doi.org/10.32583/FARMASETIS.V10I2.2002
Syachriyani, S., Arif, M., Pratiwi Agustikawati, D., Firmansyah, F., & Muhammad Farid, A. (2023). Potensi Imunomodulator Kombinasi Ekstrak Kulit Buah Semangka Merah (Citrullus Lanatus) dan Ekstrak Kulit Buah Naga Merah (Hylocereus Polyrhizus) Terhadap Peningkatan Makrofag Mencit. Pharmacology And Pharmacy Scientific Journals, 2(2), 101–110. https://doi.org/10.51577/papsjournals.v2i2.468
Valdivieso-Ugarte, M., Gomez-Llorente, C., Plaza-Díaz, J., & Gil, Á. (2019). Antimicrobial, Antioxidant, and Immunomodulatory Properties of Essential Oils: A Systematic Review. Nutrients, 11(11), 2786. https://doi.org/10.3390/nu11112786
Y., M. I., JaBWar, A., Bafadal, M., Malaka, M. H., Firdayanti, F., & Sahidin, I. (2020). Aktivitas Imunomodulator Ekstrak Etanol Spons Callyspongia sp. Terhadap Fagositosis Makrofag Pada Mencit Jantan Balb/C. Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi Dan Kesehatan, 5(1), 44–55. https://doi.org/10.36387/jiis.v5i1.377
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