Compare the Effectiveness of a Peel-Off Mask and Sheet-Mask with Green Tea Extract on Dermapen-Treated Wounds on the Back Surface of Female Wistar Rats

  • Henny Hajaratul Aswat Master Study Program in Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences, Prima Indonesia University, Medan
  • Lenni Dianna Putri Master Study Program in Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences, Prima Indonesia University, Medan
  • Andri Hidayat Master Study Program in Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences, Prima Indonesia University, Medan
Keywords: Microneedling, Peel-Off Mask, Sheet-Mask, Wound Healing, Dermapen


Microneedling has recently been cited as having high efficacy, safety, and recovery rates post-treatment. This microneedling procedure has shown results that increase patient satisfaction and awareness of doctors who want minimal treatment but still get maximum results for healing uneven skin on the face and acne scarring. The test and analysis of the difference in effectiveness between the use of a peel-off mask and a sheet mask in healing wounds treated with derma pen on the surface of the back skin of female Wistar rats (Rattus norvegicus). This research approach uses experimental quantitative research methods, namely an actual experiment or laboratory experimental design. They used a pre-test – post-test control group design to determine and analyze the differences or comparisons in the effectiveness of using a peel-off mask and a sheet mask for wounds treated with a derma pen on the skin surface. The research subjects were 10 female Wistar rats (Rattus norvegicus). The research procedure was the acclimation of test animals, phytochemical screening, treatment, and observation carried out on mice by administering dermapen wounds. The research data was analyzed using the SPSS program. In this study, Wistar strain white rats (Rattus norvegicus) with peel-off and sheet masks healed their wounds. Dermapen wounds heal after 14 days, as the average rat wound length shows. The peel-off mask group healed 100% on day 13, while the sheet-mask group healed 100% on day 12. Based on this percentage difference, the researchers found that the group with the fastest wound healing used a 12-day green tea sheet mask.


Alster, T. S., & Graham, P. M. (2018). Microneedling: A review and practical guide. Dermatologic Surgery, 44(3), 397–404.

Amer, M., Farag, F., Amer, A., ElKot, R., & Mahmoud, R. (2018). Dermapen in the treatment of wrinkles in cigarette smokers and skin aging effectively. Journal of Cosmetic Dermatology, 17(6), 1200–1204.

Asadi, S. Y., Parsaei, P., Karimi, M., Ezzati, S., Zamiri, A., Mohammadizadeh, F., & Rafieian-kopaei, M. (2013). Effect of green tea (Camellia sinensis) extract on healing process of surgical wounds in rat. International Journal of Surgery, 11(4), 332–337.

Bhatia, E., Kumari, D., Sharma, S., Ahamad, N., & Banerjee, R. (2022). Nanoparticle platforms for dermal antiaging technologies: Insights in cellular and molecular mechanisms. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, 14(2).

Chalabi, Q. S. Al, Malah, M. N. Al, & Badrani, H. B. Al. (2020). Rejuvenation of severe acne scars by microneedling. European Journal of Molecular and Clinical Medicine, 7(9), 1966–1973.

Eve Sonenblum, S., Patel, R., Phrasavath, S., Xu, S., & Bates-Jensen, B. M. (2023). Using technology to detect erythema across skin tones. Advances in Skin and Wound Care, 36(10), 524–533.

Gowda, A., Healey, B., Ezaldien, H., & Merati, M. (2021). A Systematic Review Examining the Potential Adverse Eff ects of Microneedling. Journal of Clinical and Aesthetic Dermatology, 14(1), 45–54.

Graça, M. F. P., Miguel, S. P., Cabral, C. S. D., & Correia, I. J. (2020). Hyaluronic acid—Based wound dressings: A review. Carbohydrate Polymers, 241(February), 116364.

Haydinger, C. D., Ferreira, L. B., Williams, K. A., & Smith, J. R. (2023). Mechanisms of macular edema. Frontiers in Medicine, 10(March), 1–12.

Iriarte, C., Awosika, O., Rengifo-Pardo, M., & Ehrlich, A. (2017). Review of applications of microneedling in dermatology. Clinical, Cosmetic and Investigational Dermatology, 10, 289–298.

Juhasz, M. L. W., & Cohen, J. L. (2020). Microneedling for the treatment of scars: An update for clinicians. Clinical, Cosmetic and Investigational Dermatology, 13, 997–1003.

Kang, C., & Lu, D. (2022). Combined Effect of Microneedling and Platelet-Rich Plasma for the Treatment of Acne Scars: A Meta-Analysis. Frontiers in Medicine, 8(February).

Kendall, L. V., Owiny, J. R., Dohm, E. D., Knapek, K. J., Lee, E. S., Kopanke, J. H., Fink, M., Hansen, S. A., & Ayers, J. D. (2018). Replacement, Refinement, and Reduction in Animal Studies With Biohazardous Agents. ILAR Journal, 59(2), 177–194.

Long, T., Gupta, A., Ma, S., & Hsu, S. (2020). Platelet-rich plasma in noninvasive procedures for atrophic acne scars: A systematic review and meta-analysis. Journal of Cosmetic Dermatology, 19(4), 836–844.

Makhamrueang, N., Raiwa, A., Jiaranaikulwanitch, J., Kaewarsar, E., Butrungrod, W., & Sirilun, S. (2023). Beneficial Bio-Extract of Camellia sinensis var. assamica Fermented with a Combination of Probiotics as a Potential Ingredient for Skin Care. Cosmetics, 10(3).

Mohamed, M., beshay, Y. M. A., & Assaf, H. M. (2023). Microneedling with glutathione versus microneedling alone in treatment of facial melasma: Split-face comparative study. Journal of Cosmetic Dermatology, April, 1–8.

Nilforoushzadeh, M. A., Amirkhani, M. A., Zarrintaj, P., Salehi Moghaddam, A., Mehrabi, T., Alavi, S., & Mollapour Sisakht, M. (2018). Skin care and rejuvenation by cosmeceutical facial mask. Journal of Cosmetic Dermatology, 17(5), 693–702.

Notoatmodjo, S. (2022). Metodologi Penelitian Kesehatan (3rd ed.). Jakarta: Rineka Cipta

P, M., G, M., HD, C., & A, G. (2019). Beauty Mask: Market and Environment. Journal of Clinical and Cosmetic Dermatology, 3(2).

Paichitrojjana, A. (2021). Mask acne (Maskne): A new variant of acne mechanica. Journal of the Medical Association of Thailand, 104(11), 1866–1869.

Peng, G. L. (2019). Platelet-Rich Plasma for Skin Rejuvenation: Facts, Fiction, and Pearls for Practice. Facial Plastic Surgery Clinics of North America, 27(3), 405–411.

Rezvani Ghomi, E., Khalili, S., Nouri Khorasani, S., Esmaeely Neisiany, R., & Ramakrishna, S. (2019). Wound dressings: Current advances and future directions. Journal of Applied Polymer Science, 136(27), 1–12.

Saadawi, A., Esawy, A., & Mohamed, W. (2018). Microneedling and Glycolic Acid Peel for Treatment of Acne Scar; Comparative Study. Zagazig University Medical Journal, 24(1), 44–54.

Salman, H. A., & Mohammed, Z. K. (2020). Treatment of Acne Scar Using Automated Microneedling Device of 2.5 Mm versus 1.5 Mm A Split Face Study. Iraqi Postgraduate Medical Journal, 19(2), 110–118

Schoenberg, E., O'Connor, M., Wang, J. V., Yang, S., & Saedi, N. (2020). Microneedling and PRP for acne scars: A new tool in our arsenal. Journal of Cosmetic Dermatology, 19(1), 112–114.

Sitohang, I. B. S., Sirait, S. A. P., & Suryanegara, J. (2021). Microneedling in the treatment of atrophic scars: A systematic review of randomized controlled trials. International Wound Journal, 18(5), 577–585.

Sorg, H., Tilkorn, D. J., Hager, S., Hauser, J., & Mirastschijski, U. (2017). Skin Wound Healing: An Update on the Current Knowledge and Concepts. European Surgical Research, 58(1–2), 81–94.

Suwarno, B., & Nugroho, A. (2023). Kumpulan Variabel-Variabel Penelitian Manajemen Pemasaran (Definisi & Artikel Publikasi) (1st ed.). Bogor: Halaman Moeka Publishing

How to Cite
Henny Hajaratul Aswat, Lenni Dianna Putri, & Andri Hidayat. (2024). Compare the Effectiveness of a Peel-Off Mask and Sheet-Mask with Green Tea Extract on Dermapen-Treated Wounds on the Back Surface of Female Wistar Rats . Journal La Medihealtico, 5(1), 94-102.