Journal La Lifesci 2021-03-23T08:50:03+07:00 Andrew Setiawan Rusdianto Open Journal Systems <p>International <strong>Journal La </strong><strong>Lifesci</strong>&nbsp; ISSN 2721-1207 (online) and ISSN 2721-1304 (Print) includes all the areas of research activities in all fields of Life Sciences such as Agricultural, Fisheries, Earth, Environmental Science, Botany, Zoology, Microbiology, Ecology, Ethnobiology, Biodiversity And Conservation Biology, Genetics, Biochemistry , Bioinformatics, Biophysics, Biostatistics, Cytobiology Developmental Biology, Entomology, Immunology , Molecular Biology, Virology, agronomy, plant and animal breeding, agricultural economics and rural sociology,&nbsp; Veterinary science, Ornithology, Primatology, Biogeography, Histology, Marine biology, Biochemical Sciences Aquaculture, Fishery Hydrography, Fishery Engineering, Aquatic ecosystem, Fish farming, Fisheries management, Fishery Biology, Wild fisheries, Ocean fisheries, and all fields related to life sciences.</p> Use of Antenatal Services among Women in Farming Communities in Kogi State 2021-03-22T16:25:02+07:00 Muhammed K. Ibrahim Ezekiel Haruna Safiyat E. Tanko <p>This study examined the use of antenatal services among women in farming communities in Kogi State, Nigeria. A multi-stage sampling technique was employed to collect primary data from 150 randomly selected farmers through the use of structured questionnaire. The data was analysed using descriptive statistics and logit regression model. Analysis of socio-economic variable revealed that majority (73.4%) of the respondents were below the age 40 years, with a mean household size of 6 members. About 94.7% of the respondents used antenatal care services covering an average distance of 4km in getting to nearest health facility. The study also revealed that majority (92.7%) of the respondents were aware of healthcare facilities availability and they mostly got their information from family members and friends. The use of antenatal care services among women was high and majority (95.3%) of the respondents had the knowledge of where antenatal centre is located. Also majority of the respondents visited those centres in their last pregnancy with frequency of visit between 1- 4 times. Previous visits, nearness to ANC centres and formal education positively influence the use of ANC services among women in the study area. High cost of antenatal services and poor level of information were found to be the most significant challenges facing women access to antenatal care services. &nbsp;We recommend that government should subsidize health care services for women in order to improve their affordability.</p> 2021-02-23T00:00:00+07:00 Copyright (c) 2021 Journal La Lifesci Physical Mechanical Properties of Biodegradable Plastics from Cassava Starch with Variation of Bagasse and Glycerol. 2021-03-22T10:49:00+07:00 Triana Lindriati Andrew Setiawan Rusdianto Bustani Pakartiko Firda Ainia Adha <p><em>Environmental problems are related to plastic waste that difficult to degrade naturally. Polysaccharides in the form of gelatinized cassava starch and bagasse containing 52.70% cellulose can replace commercial plastic polymers. This research was aimed to determine the effect of variations in bagasse and glycerol on physical, mechanical and biodegradation tests. The study used 2 factors CRD consisting of the addition of bagasse and glycerol. The results showed that the addition of bagasse and glycerol reducing the brightness with a value range of 44.82-76.15 and increased absorption between 2.61-10.64%. The thickness occurred between 0.79-1.11mm. The tensile strength values are 8.30-14.33 MPa, inversely proportional to the elongation value between 11.85-94.22%, but directly proportional to the modulus young value between 0.09-1.24 MPa. The highest biodegradation values were 0% bagasse and 40% glycerol. The three best treatments were found in the combination treatment of bagasse and glycerol 0% 20%, 0% 30%, and 0% 40%.</em></p> 2021-02-23T13:16:24+07:00 Copyright (c) 2021 Journal La Lifesci Genome Editing of Saccharomyces Cerevisiae Using CRISPR-Cas9 System 2021-03-23T08:50:03+07:00 Yaseen Ismael Imran Ibrahim Abdulla Ahmed Ahmed Ali Mhawesh <p><em>Saccharomyces cerevisiae </em>is an important yeast has been exploited for a long time to produce alcohol or bread. Moreover, genetically engineered <em>S. cerevisiae</em> cells continue to be used as cell factories for production of biofuels, pharmaceutical proteins and food additives. Genetically modified strain of <em>S. cerevisiae</em> created using traditional methods is laborious and time consuming. Recently, originally an immune system in archaea and bacteria, Clustered regularly interspaced short palindromic repeats “CRISPR” and CRISPR-associated “Cas” have been used exploited&nbsp; as a flexible tool for genome editing. Until now, this tool has been applied to many organisms including yeast. Here, we review the importance of <em>S. cerevisiae</em> as an industrial platform and the use of CRISPR/Cas system and its applications in research and industry of this yeast.</p> <p>&nbsp;</p> 2021-03-23T08:50:03+07:00 Copyright (c) 2021 Journal La Lifesci